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A horse chestnut extract, which induces contraction forces in fibroblasts, is a potent anti‐aging ingredient
Author(s) -
Fujimura Tsutomu,
Tsukahara Kazue,
Moriwaki Shigeru,
Hotta Mitsuyuki,
Kitahara Takashi,
Takema Yoshinori
Publication year - 2007
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1467-2494.2007.00369_3.x
Subject(s) - contraction (grammar) , aesculus hippocastanum , fibroblast , ingredient , chemistry , myofibroblast , wound healing , horse , stress fiber , dermal fibroblast , active ingredient , anatomy , cell , pharmacology , biology , surgery , medicine , pathology , cytoskeleton , biochemistry , botany , endocrinology , food science , paleontology , in vitro , fibrosis
Contraction forces generated by non‐muscle cells, such as fibroblasts, play important roles in determining cell morphology, vasoconstriction, and/or wound healing. We have searched among various plant extracts for ingredients that generate cell contraction forces using fibroblast‐populated collagen gels. Using that model, we found that an extract of horse chestnuts ( Aesculus hippocastanum ) is able to generate such contraction forces in fibroblasts. The involvement of stress fiber formation in that response is suggested by the inhibition of such force generation by cytochalasin D and rhodamine phalloidin stain. Clinical testing of the extract was carried out using 40 healthy female volunteers. A gel formulation that included 3% of the extract was applied topically to the skin around the eye three times daily for 9 weeks. The efficacy of the extract to diminish wrinkles was evaluated by visual scoring based on photo scales. After 6 weeks, significant decreases in the wrinkle scores at the corners of the eye or in the lower eyelid skin were observed compared with controls. After 9 weeks, similar results were obtained. Taken together, our results suggest that an extract of horse chestnuts can generate contraction forces in fibroblasts and is a potent anti‐aging ingredient.