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Production of the Tissue Inhibitor of Metalloproteinase‐2 (TIMP‐2) and Evaluation of Its Potential as Anti‐Aging Active
Author(s) -
Schütz R.,
König B.,
Hochstrasser B.,
Stolz P.,
Imfeld D.
Publication year - 2006
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1467-2494.2006.00300_2.x
Subject(s) - matrix metalloproteinase , extracellular matrix , skin aging , chemistry , in vitro , endogeny , tissue inhibitor of metalloproteinase , enzyme , metalloproteinase , wrinkle , human skin , recombinant dna , biochemistry , microbiology and biotechnology , pharmacology , medicine , biology , dermatology , gene , gerontology , genetics
Collagen‐degrading matrix metalloproteinases (MMPs) show increased activities predominantly in UV‐irradiated skin as well as in chronologically aged skin. MMPs are therefore reasoned to be associated with the degenerative alterations in the collagenous dermal extracellular matrix, which in turn may accelerate the signs of skin aging such as wrinkle formation and skin sagging. In normal physiological processes MMP activity is controlled by the endogenous tissue inhibitors of metalloproteinases (TIMPs). Here we report the biotechnological production of the human‐identical inhibitor TIMP‐2 and its evaluation as an anti‐aging active through attenuation of the degenerative MMP activity. The recombinant TIMP‐2 polypeptide selectively inhibited MMPs in vitro which are involved in aging processes and showed efficient IC 50 values in the low nanomolar range. Topical application of formulated TIMP‐2 on skin explants with subsequent UVB irradiation (2‐3 MED) resulted in an almost quantitative protection against collagen degradation. Thus, our biotechnological production of TIMP‐2 leads to the active broad‐spectrum MMP inhibitor that reduces excessive MMP activity and thereby preserves the structural integrity of collagen network. A poster of this work was presented at the 23rd IFSCC Congress 2004, Orlando, Florida, USA.

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