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A simple and specific high performance liquid chromatography method for the assay of a series of novel dermal penetration enhancers
Author(s) -
MICHNIAK B. B.,
SEYDA K. L.
Publication year - 1993
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1467-2494.1993.tb00063.x
Subject(s) - chromatography , series (stratigraphy) , simple (philosophy) , penetration (warfare) , chemistry , mathematics , biology , philosophy , operations research , epistemology , paleontology
Synopsis A series of clofibric acid amides has been synthesized and previously reported by the authors as possessing enhancer activity in vitro in athymic nude mouse skin against model drugs, hydrocortisone‐21‐acetate and β‐methasone‐17‐valerate. An assay was required for each of these enhancers however, which would be specific for each compound and would also separate model drugs and their metabolite peaks. This study reports reverse phase high performance liquid chromatography assays for clofibric acid amide and seven derivatives (Ia–Ig). All enhancers showed maximum absorption at 232 nm, betamethasone (BM) and its valerate (BMV) at 238 nm, and hydrocortisone (HC) and its acetate (HCA) at 242 nm. Practical units of detection for the amides were 0.46–2.8 μg ml ‐1 and peaks were sharp and well‐separated from steroid peaks in three vehicles – methanol alone. Franz diffusion cell receptor phase samples (isotonic phosphate buffer), and full‐thickness athymic nude mouse skin extracts in methanol. Mobile phases consisted of various proportions of acetonitrile and water, some with 2‐propanol. The octyl amide for example, with mobile phase CH 3 CN: H 2 O (85:15) at 1 ml min ‐1 had a retention time (t R ) of 7.9 mins. Under the same conditions, retention times for the steroids were HC, t R = 3.3 mins; HCA, t R = 4.3 mins; BM, t R = 3.4 mins; BMV, t R = 4.6 mins.