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Telomere shortening occurs in Asian Indian Type 2 diabetic patients
Author(s) -
Adaikalakoteswari A.,
Balasubramanyam M.,
Mohan V.
Publication year - 2005
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.2005.01574.x
Subject(s) - tbars , medicine , diabetes mellitus , telomere , type 2 diabetes , endocrinology , malondialdehyde , type 2 diabetes mellitus , thiobarbituric acid , lipid peroxidation , oxidative stress , biology , genetics , gene
Aim Telomere shortening has been reported in several diseases including atherosclerosis and Type 1 diabetes. Asian Indians have an increased predilection for Type 2 diabetes and premature coronary artery disease. The aim of this study was to determine whether telomeric shortening occurs in Asian Indian Type 2 diabetic patients. Methods Using Southern‐blot analysis we determined mean terminal restriction fragment (TRF) length, a measure of average telomere size, in leucocyte DNA. Type 2 diabetic patients without any diabetes‐related complications ( n = 40) and age‐ and sex‐matched control non‐diabetic subjects ( n = 40) were selected from the Chennai Urban Rural Epidemiology Study (CURES). Plasma level of malondialdehyde (MDA), a marker of lipid peroxidation, was measured by TBARS (thiobarbituric acid reactive substances) using a fluorescence method. Results Mean (± SE) TRF lengths of the Type 2 diabetic patients (6.01 ± 0.2 kb) were significantly shorter than those of the control subjects (9.11 ± 0.6 kb) ( P = 0.0001). Among the biochemical parameters, only levels of TBARS showed a negative correlation with shortened telomeres in the diabetic subjects ( r = −0.36; P = 0.02). However, telomere lengths were negatively correlated with insulin resistance (HOMA‐IR) ( r = −0.4; P = 0.01) and age ( r = −0.3; P = 0.058) and positively correlated with HDL levels ( r = 0.4; P = 0.01) in the control subjects. Multiple linear regression (MLR) analysis revealed diabetes to be significantly ( P < 0.0001) associated with shortening of TRF lengths. Conclusions Telomere shortening occurs in Asian Indian Type 2 diabetic patients.