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The TGF‐β1 gene codon 10 polymorphism contributes to the genetic predisposition to nephropathy in Type 1 diabetes
Author(s) -
Patel A.,
Scott W. R.,
Lympany P. A.,
Rippin J. D.,
Gill G. V.,
Barnett A. H.,
Bain S. C.
Publication year - 2005
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.2005.01376.x
Subject(s) - diabetic nephropathy , medicine , nephropathy , genetic predisposition , genotype , type 2 diabetes , genotyping , diabetes mellitus , type 1 diabetes , endocrinology , genetics , gene , biology , disease
Aims We hypothesize that transforming growth factor‐beta (TGF‐β), a multifunctional growth factor which plays a key role in the development of tissue fibrosis, may be involved in the pathophysiology of diabetic nephropathy. Our aim was to examine three polymorphisms within the TGF‐β1 gene, in codons 10, 25 and 263, for association with nephropathy in Type 1 diabetes. Methods We conducted a large case–control study using cases with Type 1 diabetes and clinical nephropathy. Controls were Type 1 diabetic subjects who have been injecting insulin for at least 50 years and have extremely low risk of nephropathy. Genotyping was by polymerase chain reaction with sequence‐specific primers. Results There was a significant difference in the frequency of the TGF‐β1 codon 10 genotypes in the diabetic nephropathy group ( n = 420) when compared with the controls ( n = 410, P = 0.007). There were no significant differences when the frequencies of the TGF‐β1 codons 25 and 263 genotypes in the diabetic nephropathy group were compared with the control group. Conclusions In our study the TGF‐β1 codon 10 polymorphism is associated with nephropathy in Type 1 diabetes and variation in this gene may contribute to the genetic predisposition to this complication in Type 1 diabetes.