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Human Red Cell Membrane Fluidity and Calcium Pump Activity in Normolipidaemic Type II Diabetic Subjects
Author(s) -
Muzulu S.I.,
Bing R.F.,
Norman R.I.,
Burden A.C.
Publication year - 1994
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1994.tb00350.x
Subject(s) - medicine , membrane fluidity , calcium , membrane , endocrinology , biophysics , biochemistry , chemistry , biology
Red cell membrane cholesterol, 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) and 1‐[(4‐trimethylammonium)phenyl]‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) anisotropics and basal and calmodulin‐stimulated calcium pump activities were compared in 16 normolipidaemic Type 2 (non‐insulin‐dependent) diabetic patients and 20 normolipidaemic control subjects using the Mann‐Whitney U‐test. Serum cholesterol, membrane cholesterol, and membrane DPH and TMA‐DPH anisotropics were similar in the two groups but both basal and calmodulin‐stimulated calcium pump activities were reduced in the diabetic group: basal activity (median (inter‐quartile range), μmol mg −1 h −1 ) 1.66 (1.18–1.97) vs 2.09 (1.90–2.50), p < 0.005 and calmodulin‐stimulated activity 4.19 (3.07–5.48) vs 5.53 (4.70–6.88), p < 0.006. Although there were no correlations between glycaemic control and membrane anisotropy and between glycaemic control and calcium pump activity, the reduction in calcium pump activity is most likely due to a direct effect of diabetes on the calcium pump protein itself.