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A Comparison of Different Methods of Assessing Free Radical Activity in Type 2 Diabetes and Peripheral Vascular Disease
Author(s) -
MacRury S.M.,
Gordon D.,
Wilson R.,
Bradley H.,
Gemmell C.G.,
Paterson J.R.,
Rumleyd A.G.,
MacCuish A.C.
Publication year - 1993
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1993.tb00074.x
Subject(s) - medicine , diabetes mellitus , superoxide dismutase , ceruloplasmin , chemiluminescence , endocrinology , type 2 diabetes , biochemistry , oxidative stress , chemistry , chromatography
Increased free radical activity in diabetes mellitus may contribute to the higher prevalence and mortality from macrovascular disease in diabetic patients. To investigate this, levels of plasma antioxidants (superoxide dismutase, caeruloplasmin, plasma, and lysate thiol), diene conjugates, lipid peroxides, and chemiluminescence were measured in diabetic and non‐diabetic patients with peripheral vascular disease compared with healthy control subjects. Caeruloplasmin, diene conjugate ratio, and lipid peroxides were significantly increased in patients with vascular disease but there was no difference between diabetic and non‐diabetic patients. Conjugated diene ratio correlated with caeruloplasmin ( r = 0.40, p < 0.02) and inversely with superoxide dismutase level ( r = −0.36, p < 0.05) but there was no significant correlation between other antioxidants and diene conjugates, lipid peroxides or chemiluminescence. The relationship between different indirect measurements of free radical activity is variable but there appears to be no additive effect of diabetes on the increased free radical activity associated with vascular disease.