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Neutrophil Aldose Reductase Activity and its Association with Established Diabetic Microvascular Complications
Author(s) -
Dent M. T.,
Tebbs S. E.,
Gonzalez A. M.,
Ward J. D.,
Wilson R. M.
Publication year - 1991
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1991.tb01628.x
Subject(s) - medicine , aldose reductase , diabetes mellitus , dermatology , endocrinology
Direct investigation of the polyol pathway is rarely possible in studies of human diabetes. A spectrophotometric assay has been developed for the measurement of aldose reductase and sorbitol dehydrogenase activity in the neutrophil. Neutrophil aldose reductase activity was increased in patients with Type 1 diabetes with complications (median 40 (interquartile range 28–48) u, where 1 unit of enzyme activity = nmol NADPH min −1 10 8 ‐cells −1 ) compared with those without complications (20 (16–36) u, p < 0.01) and normal control subjects (20 (8–36) u, p < 0.01). In Type 2 diabetes, patients with complications also had higher aldose reductase activity (40 (28–52) u) than those without complications (24 (16–36) u, p < 0.01). There were no differences between patients without complications and normal control subjects. Sorbitol dehydrogenase activity was decreased in diabetic patients ( p < 0.02) but not significantly different between diabetic patients with and without complications.