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Reduced Free Radical Activity Detected by Decreased Diene Conjugates in Insulin‐dependent Diabetic Patients
Author(s) -
Collier A.,
Jackson M.,
Dawkes R. M.,
Bell D.,
Clarke B. F.
Publication year - 1988
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1988.tb01101.x
Subject(s) - medicine , radical , linoleic acid , endocrinology , diene , diabetes mellitus , lipid peroxide , diabetic retinopathy , insulin , conjugated linoleic acid , biochemistry , chemistry , fatty acid , lipid peroxidation , oxidative stress , organic chemistry , natural rubber
Free radicals are unstable chemical species which react with and oxidize adjacent molecules, particularly polyunsaturated lipids. The diene‐conjugated non‐peroxide isomer of linoleic acid (PL‐9,11‐LA') has been identified as the main diene‐conjugated compound in plasma, and is a probable marker of free radical activity. The aim of the current study was to determine whether the level of PL‐9,11‐LA', measured by HPLC, is altered in insulin‐dependent diabetes, and to investigate whether any abnormality demonstrated correlated with microvascular disease in the form of retinopathy. There was no difference in the concentrations of linoleic acid between the diabetic and control groups (422(129) vs 402(81) (SD) μmol l −1 ). However, the concentration of PL‐9,11‐LA' was significantly reduced in the diabetic group compared with control group (15.6(6.7) vs 19.3(3.9) μmol l −1 , p <0.01), with the molar ratio of PL‐9,11‐LA':Iinoleic acid x 100 similarly reduced (3.8(1.3) vs 5.0(1.6)%, p <0.005). This study does not support the concept that free radicals play a significant role in the development of diabetic vascular disease.