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Detection of Antibodies against Wheat Germ Agglutinin Bound Glycoproteins on the Islet‐cell Membrane
Author(s) -
Kitano N.,
Taminato T.,
Ida T.,
Seno M.,
Seino Y.,
Matsukura S.,
Kuno S.,
Imura H.
Publication year - 1988
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1988.tb00960.x
Subject(s) - antibody , wheat germ agglutinin , islet , glycoprotein , medicine , immunology , nephelometry , immunofluorescence , agglutinin , microbiology and biotechnology , endocrinology , diabetes mellitus , biology , lectin
An attempt was made to detect islet cell surface antibodies (ICSAb) using solubilized islet‐cell glycoproteins as antigens. Isolated rat islets were labelled with 125 l‐wheat germ agglutinin (WGA) and solubilized by Nonidet P‐40 with sonication. 125 l‐WGA‐bound islet‐cell proteins were incubated with test sera, and bound antibodies were precipitated with anti‐human IgG or IgM immunobeads. Serum which had a bound percent beyond the mean plus 2SD of control sera was defined as antibody‐positive. Results obtained by this method correlated well with those by the immunofluorescence method of detecting ICSAb. Prevalences of antibodies were 21/114 (18%) for IgG antibodies and 9/114 (8%) for IgM antibodies in patients with insulin‐dependent diabetes mellitus (IDDM). The prevalence was highest for both IgG and IgM antibodies in patients within a year of the onset of disease (38 and 25%, respectively), and decreased thereafter. The prevalence of IgM antibodies was lower than that of IgG antibodies at all stages. In NIDDM patients, the prevalence of antibodies was 5/72 (7%) for both IgG and IgM antibodies. If these preliminary results are confirmed, this radioassay may be developed to detect antibodies against islet cell membrane proteins on a large scale.