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A Genetic Analysis of Type 2 (Non‐insulin‐dependent) Diabetes Mellitus in Punjabi Sikhs and British Caucasoid Patients
Author(s) -
Hitman G. A.,
Karir P. K.,
Mohan V.,
Rao P. V.,
Kohner E. M.,
Levy J. C.,
Mather H.
Publication year - 1987
Publication title -
diabetic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.474
H-Index - 145
eISSN - 1464-5491
pISSN - 0742-3071
DOI - 10.1111/j.1464-5491.1987.tb00923.x
Subject(s) - insulin , medicine , diabetes mellitus , hypervariable region , restriction fragment length polymorphism , human leukocyte antigen , endocrinology , type 2 diabetes mellitus , insulin receptor , gene , genetics , polymerase chain reaction , antibody , biology , immunology , insulin resistance , antigen
A genetic analysis of diabetic and non‐diabetic Punjabi Sikhs ( n = 164) was made for markers of non‐insulin‐dependent diabetes mellitus using insulin receptor, insulin, and HLA‐D αchain gene probes. Additionally British Caucasoids ( n =163) were studied using the insulin receptor probe. Insulin receptor gene restriction fragment length polymorphisms were defined using Southern blot techniques and the restriction enzyme Bgl II and Bam HI. In Punjabi Sikhs and British Caucasoids neither of the restriction fragment length polymorphisms distinguished non‐insulin‐dependent diabetes mellitus subjects from controls. In the Sikhs no association with non‐insulin‐dependent diabetes mellitus was seen with the hypervariable region of the insulin gene or with HLA‐DR/DQ/DXα chain restriction fragment length polymorphisms. We therefore conclude that despite the high prevalence of non‐insulin‐dependent diabetes mellitus in Asians we were unable to find any genetic markers for this disease using the available cloned gene probes.