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Urinary fluorescence in situ hybridization assay for detecting urothelial carcinoma in Taiwanese patients
Author(s) -
Chuang KunLung,
Chuang HunChang,
Ng KwaiFong,
Chang YingHsun,
Wu ChunTe,
Chuang ChengKeng,
Liao ShuenKuei,
Pang SeeTong
Publication year - 2010
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/j.1464-410x.2009.08917.x
Subject(s) - fluorescence in situ hybridization , bladder cancer , cytology , upper urinary tract , urinary system , urology , pathology , carcinoma in situ , aneuploidy , medicine , carcinoma , urinary bladder , biology , cancer , chromosome , genetics , gene
Study Type – Diagnosis (case series)
Level of Evidence 4 OBJECTIVES To investigate the cytogenetic marker detected by fluorescence in situ hybridization (FISH; UroVysion TM , Vysis, Inc., Abbott Laboratories, Des Plaines, IL, USA) in the diagnosis of bladder cancer and upper tract urothelial carcinoma (UC) in Taiwanese patients, as FISH has been used in Western countries for detecting UC, but there are limited results in Asian populations. PATIENTS AND METHODS We analysed polyploidy of chromosome 3, 7, 17 and aneuploidy of chromosome 21, using uroepithelial cells collected at the first void or by instrumental extraction of urine, for bladder cancer, and shedding cells from the upper tract flushed by normal saline via ureteric catheterization or ureterorenoscopy. The criteria for positive tumour cells included three or more positive staining in two or more chromosomes showing polyploidy or <50% staining of the chromosome 9p21. RESULTS In all, 32 patients with bladder UC and 12 with upper tract UC were assessed. The overall sensitivity for bladder cancer by UroVysion was 96.8%. The sensitivity of the cytology test was 36% for UC of the bladder. The sensitivity for UroVysion in upper tract UC was 12/12 but the specificity was three of nine. CONCLUSIONS This preliminary report shows that UroVysion was a sensitive screening method for UC of the bladder and upper urinary tract.