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The effect of endothelin‐1 on the production of interleukin‐6 in cultured human detrusor smooth muscle cells, and the effect of interleukin‐6 on the contractile response of bladder smooth muscle strips from rats
Author(s) -
Han June Hyun,
Lee Moo Yeol,
Myung Soon Chul
Publication year - 2009
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/j.1464-410x.2009.08465.x
Subject(s) - endocrinology , medicine , chemistry , contraction (grammar) , bethanechol , endothelin 1 , detrusor muscle , acetylcholine , sodium nitroprusside , endothelin receptor , urinary bladder , biology , muscarinic acetylcholine receptor , nitric oxide , receptor
OBJECTIVE To determine the effect of endothelin‐1 (ET‐1) on the production of interleukin‐6 (IL‐6) in cultured human detrusor smooth muscle cells (HDSMCs) and the effect of IL‐6 on the contractile response of bladder smooth muscle strips from rats. MATERIALS AND METHODS The expression of IL‐6 mRNA and production of IL‐6 protein in cultured HDSMCs treated with the different concentrations of ET‐1 were assayed by reverse transcriptase‐polymerase chain reaction and specific enzyme‐linked immunosorbent assay, respectively. The strips from Sprague‐Dawley rats were either untreated or treated with 1 ng/mL of IL‐6 for 60 min. Using increasing cumulative concentrations of acetylcholine (ACh), bethanechol (BCh), or sodium nitroprusside, we assessed the concentration‐contraction or the relaxation responses. In cystitis‐induced strips, change of contractions induced by noradrenaline (NA) with or without treatment of IL‐6 were assessed. The IL‐6‐treated strips were incubated for 30 min in the presence or absence indomethacin or SQ29548, and then the effects on ACh‐ or BCh‐induced contractions were measured. RESULTS The expression of IL‐6 mRNA and the production of IL‐6 protein on the cultured HDSMCs pretreated by ET‐1 were significantly higher than in the control ( P < 0.05). The ACh‐ and BCh‐induced contractions were increased in the IL‐6 pretreated strips from both dome and trigone, regardless of the presence of urothelium ( P < 0.05). The presence of cystitis augmented the NA‐induced contractions ( P < 0.05). The contractions induced by ACh and BCh were inhibited by indomethacin and SQ29548. CONCLUSIONS ET‐1 induces expression of IL‐6 mRNA and production of IL‐6 protein on HDSMCs. IL‐6 enhances detrusor smooth muscle contractility via the muscarinic or adrenergic receptor pathway.