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Antitumour effect of electrochemotherapy with bleomycin on human prostate cancer xenograft
Author(s) -
Ueki Teiichiro,
Uemura Hiroji,
Nagashima Yoji,
Ohta Shinsuke,
Ishiguro Hitoshi,
Kubota Yoshinobu
Publication year - 2008
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/j.1464-410x.2008.07793.x
Subject(s) - bleomycin , electrochemotherapy , electroporation , medicine , apoptosis , prostate cancer , prostate , cancer research , cancer , chemotherapy , chemistry , biochemistry , gene
OBJECTIVE To investigate the antitumour effect of electroporation (EP), a drug delivery system that has been shown to be effective synergistically with antitumour drugs, with bleomycin on the growth of prostate cancer xenografts in nude mice. MATERIALS AND METHODS PC‐3 cells were implanted subcutaneously into nude mice. After determination of the optimal conditions of electric pulse voltage and bleomycin dose, tumour growth in mice treated with EP plus bleomycin was compared with that in mice receiving EP alone, bleomycin alone or no treatment. In all four groups, apoptosis in the tumours was assessed. RESULTS There was a significant reduction in tumour growth in mice that received EP with bleomycin. Apoptotic cells in tumours at 24 h after treatment with EP plus bleomycin showed a significant difference compared with the other three groups, but not at 12 h after treatment. CONCLUSIONS These results indicate the possibility that EP with bleomycin could be effective as an ablation therapy for prostate cancer, especially androgen‐independent cancer.