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Effects of a vitamin D 3 analogue in a rat model of bladder outlet obstruction
Author(s) -
SCHRÖDER ANNETTE,
COLLI ENRICO,
MAGGI MARIO,
ANDERSSON KARLERIK
Publication year - 2006
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/j.1464-410x.2006.06342.x
Subject(s) - cystometry , urination , bladder outlet obstruction , medicine , contractility , muscle hypertrophy , urology , endocrinology , vitamin , urinary bladder neck obstruction , prostate , stimulation , calcitriol receptor , urinary bladder , vitamin d and neurology , urinary system , cancer
OBJECTIVES To explore the effect of the vitamin D 3 analogue, BXL‐628, on some of the consequences of bladder outlet obstruction (BOO), e.g. hypertrophy and loss of contractile function, as vitamin D 3 and BXL‐628 inhibit prostate and bladder cell growth in vitro , and there are receptors for vitamin D in rat and human bladder. MATERIAL AND METHODS In female rats, BOO was produced by a standardized method; one group received daily BXL‐628 (150 µg/kg per day) and the remaining rats received vehicle. Sham‐operated rats received BXL‐628 or vehicle. After 2 weeks, the conscious rats were assessed by cystometry. Plasma calcium levels were determined and in vitro contractility assessed at the end of the experiments. RESULTS There was a significant increase in bladder weight, micturition interval and volume, and in bladder capacity in both the obstructed groups compared to sham controls, but no difference between the obstructed groups. On plotting the micturition pressure against bladder weight within the obstructed groups, there was a clear correlation in the vehicle‐treated group, indicating a decrease in contractile function with increasing bladder weight. There was no such correlation in the treatment group. In vitro , there was a strong correlation of increasing bladder weights vs decrease in response to KCl and electrical‐field stimulation in strips from obstructed vehicle‐treated rats, but no correlation in those from drug‐treated rats. Treatment increased the plasma calcium level by 12%. CONCLUSIONS The vitamin D 3 analogue used did not prevent bladder hypertrophy, but appeared to reduce some of the negative functional changes of the bladder smooth muscle, which occurs with BOO‐induced increases in bladder weight.