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Protective effects of grape suspension on in vivo ischaemia/reperfusion of the rabbit bladder
Author(s) -
Lin Alpha DianYu,
Mannikarottu Anita,
Chaudhry Ahsen,
Whitbeck Catherine,
Kogan Barry A.,
Chichester Paul,
Levin Robert M.
Publication year - 2005
Publication title -
bju international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 1464-4096
DOI - 10.1111/j.1464-410x.2005.05832.x
Subject(s) - ischemia , medicine , in vivo , lagomorpha , anesthesia , reperfusion injury , stimulation , urology , endocrinology , biology , microbiology and biotechnology
OBJECTIVE To investigate the potential protective effect of a grape suspension in a rabbit model of in vivo bilateral ischaemia/reperfusion (I/R), which is a causal factor in obstructive bladder dysfunction. MATERIALS AND METHODS Six groups of four New Zealand White rabbits were treated by twice‐daily gavage with aqueous grape suspension (groups 1–3) or sugar‐water vehicle (groups 4–6) for 3 weeks. Groups 1 and 4 then received bilateral ischaemia for 2 h, and groups 2 and 5 received bilateral ischaemia for 2 h and reperfusion (recovery) for 1 week. Groups 3 and 6 were controls (sham‐operated). The effects on cystometry, in vitro contractile responses, and morphology were evaluated. RESULTS Ischaemia resulted in significant reductions in the contractile responses to all forms of stimulation in vehicle‐fed rabbits, whereas there were no reductions in grape‐fed rabbits. Contractile responses were significantly reduced in both I/R groups, but significantly more in vehicle‐fed than in grape‐fed rabbits. Immunohistochemical studies showed less hypoxia in the bladders of grape‐fed rabbits than in vehicle‐fed rabbits for both ischaemia‐only and I/R groups. CONCLUSIONS Feeding rabbits with grape suspension provided significant protection against the hypoxic effects of bilateral ischaemia and I/R.