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Electrolyte homeostasis in urothelial neoplasia: the effects of amiloride
Author(s) -
Cartman M.L.,
Morris J.A.,
Huddart H.,
Staff W.G.
Publication year - 1995
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1995.tb07416.x
Subject(s) - amiloride , urothelium , incubation , chemistry , homeostasis , saline , urothelial cell , intracellular , neoplastic cell , electrolyte , sodium , endocrinology , cell , medicine , biochemistry , biology , urinary bladder , organic chemistry , electrode
Objective To investigate the effect of amiloride pre‐incubation on the intracellular electrolyte levels in neoplastic and non‐neoplastic urothelium. Materials and methods Samples of papillary transitional cell carcinomas (grades 1 and 2) and non‐neoplastic human urothelium were either frozen immediately or after pre‐incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo‐ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X‐ray microanalysis. Results In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl‐ were significantly increased in neoplastic compared with non‐neoplastic urothelial cells ( P <0.001). This difference was also found in the cells pre‐incubated with Krebs saline alone but was not present in the cells pre‐incubated with amiloride. Conclusions These results were consistent with the concept that activation of the amiloride‐sensitive Na+/H + membrane exchanger is a primary event in neoplasia.