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Numerical chromosome aberrations in bladder cancer detected by in situ hybridization
Author(s) -
Nemoto R.,
Nakamura I.,
Uchida K.,
Harada M.
Publication year - 1995
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1995.tb07267.x
Subject(s) - bladder cancer , urinary bladder , biology , in situ hybridization , chromosome , interphase , cytogenetics , pathology , stage (stratigraphy) , in situ , transitional cell carcinoma , pathological , aneuploidy , comparative genomic hybridization , cancer , karyotype , carcinoma in situ , medicine , genetics , chemistry , gene , paleontology , gene expression , organic chemistry
Objective To investigate the relationship between interphase cytogenetics and the grade and stage of bladder Cancer in patients with transitional cell carcinomas of the urinary bladder. Patients and methods By use of in situ hybridization with chromosome‐specific DNA probes, the copy number of pericentromeric sequences on chromosomes 7, 10, 11, 17, 18, X and Y was detected within interphase nuclei in formalin‐fixed and paraffin‐embedded sections of the routinely processed bladder cancers from 20 patients. The percentage of hyperdi‐ploid cells (three or more spots) was estimated using light microscopy. Results The percentage of hyperdiploid cells for chromosomes 7, 11 and 17 was highly correlated with increasing tumour grade ( P <0.01, Spearman rank correlation) or increasing pathological stage ( P <0.01). The percentage of hyperdiploid cells for chromosome Y was not correlated with either grade or stage ( P >0.05). As high tumour grade and stage are both indicative of more aggressive tumour behaviour and a worse prognosis, these findings suggest that the percentage of hyperdiploid cells, especially for chromosomes 7,11 and 17, may be highly predictive of bladder tumour aggressiveness. Conclusion These preliminary results suggest that measurement of numerical chromosome aberrations using in situ hybridization in bladder cancer may offer a new objective and quantitative assay of the biological potential of individual tumours.