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Immunohistochemical Detection of Tissue‐infiltrating Lymphocytes in Mild Urothelial Dysplasia
Author(s) -
MIGLIARI R.,
DEMIRY *M. I. M. EL,
MUSCAS G.,
MELIS M.,
USAI E.
Publication year - 1993
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1993.tb16290.x
Subject(s) - urothelium , immunoperoxidase , pathology , immunohistochemistry , cytotoxic t cell , dysplasia , monoclonal antibody , antigen , monocyte , epithelium , cd68 , biology , immunology , antibody , microbiology and biotechnology , chemistry , medicine , in vitro , urinary system , anatomy , biochemistry
Summary The presence and distribution of immunocompetent cells in normal and dysplastic urothelium of the bladder were investigated in 3 patients. Using an indirect immunoperoxidase staining procedure, monoclonal antibodies reacting with B (Leu 12 +) and T (Leu4 +) cells, cells of the suppressor/cytotoxic (Leu2a +), helper/inducer (Leu3a +) and natural killer (Leu7 +) phenotypes, monocyte‐macrophages (LeuM3 +), and cells expressing interleukin‐2 receptor and HLA‐DR antigen (HLA‐DR+) were tested in all specimens. The results confirmed the presence of a well ordered and compartmentalised distribution of immunocompetent cells in the normal mucosa far from dysplasia. There was an increased number of lymphocytes in the hyperplastic/dysplastic urothelium with associated Von Brunn's nests. Leu2a + cells, HLA‐DR expressing cells and monocyte‐macrophages were present inside the epithelium, particularly within dysplasia and von Brunn's nests, but the Leu3a + /Leu2a + ratio was not inverted (0.48 + 0.1 to 0.74+ 0.2). These findings reveal that in mild urothelial dysplasia with von Brunn's nests there is a spatial modification of the immunological barrier associated with the mucosa.