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Sperm Morphological Analysis: Comparison of Two Methods in Human Semen
Author(s) -
BADENOCH D. F.,
EVANS P. R.,
GRAY A.,
EVANS S. J. W.
Publication year - 1990
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1990.tb14732.x
Subject(s) - sperm , papanicolaou stain , semen analysis , andrology , semen , azoospermia , male infertility , significant difference , morphological analysis , biology , infertility , medicine , psychology , pregnancy , genetics , cervical cancer , cancer , cognitive psychology
Summary— Sperm morphological analysis was performed by 2 methods: light microscopy on Papanicolaou stained material and scanning electron microscopy. Both methods were found to have acceptably low levels of observer error and a positive correlation existed between them. Sperm morphology was analysed by both methods in 2 groups: (1) 104 proven “fertile” men, husbands of women who had just given birth and whose paternity had been proven by HLA studies with > 95.5% certainty; (2) 53 infertile men from primary infertile marriages in whom no female cause could be found and excluding those with azoospermia. There was no statistically significant difference in medians between the 2 groups when sperm morphology was analysed by light microscopy, but analysis by scanning electron microscopy did show a significant difference.