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Immunohistochemical Staining with Monoclonal Antibody 32–2B to Desmosomal Glycoprotein 1: Its Role in the Histological Assessment of Urothelial Carcinomas
Author(s) -
CONN I. G.,
VILELA M. J.,
GARROD D. R.,
CROCKER J.,
WALLACE D. M. A.
Publication year - 1990
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1990.tb14694.x
Subject(s) - staining , pathology , immunohistochemistry , monoclonal antibody , stromal cell , antibody , biology , stroma , urothelium , medicine , immunology , anatomy , urinary system
Summary— A series of transitional cell carcinomas of bladder were stained immunohistochemically with the monoclonal antibody, 32–2B, to desmosomal glycoprotein 1. All of the sections showed positive staining with the antibody. Assessment of staining intensity, by 3 independent examiners, revealed a strong negative correlation between density of desmosomal staining and degree of invasion ( P =0.012). Nests of strongly staining cells were identified in several invasive tumours, possibly indicating early squamous differentiation. Invasive tumour cells in the subepithelial stroma also stained strongly with the antibody. Correlation with clinical course, however, revealed no significant association between desmosomal staining and the incidence of recurrence or progression. It is suggested that staining with this antibody may be of value in detecting both stromal invasion and early squamous differentiation of transitional cell carcinomas. Both this and previous studies emphasise the value of this antibody as an epithelial marker in neoplasia.