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Oncogenes and Bladder Cancer
Author(s) -
MALONE P. R.,
VISVANATHAN K. V.,
PONDER B. A. J.,
SHEARER R. J.,
SUMMERHAYES I. C.
Publication year - 1985
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1985.tb07028.x
Subject(s) - transfection , cancer research , transitional cell carcinoma , oncogene , gene duplication , gene , microbiology and biotechnology , biology , southern blot , pathogenesis , bladder cancer , cancer , immunology , genetics , cell cycle
Summary— We screened a series of 15 superficial and invasive transitional cell carcinomas of the bladder for transforming activity on the NIH/3T3 transfection assay. In addition, by obtaining tumour DNA and normal lymphocyte DNA from the same patients we looked for evidence of gene amplification or rearrangement of the c‐H‐ras‐I gene by Southern blot analysis. One patient (with a grade 2 T1 tumour) was shown to have an activated oncogene on the NIH/ 3T3 transfection assay and this was identified as a c‐H‐ras‐I gene. We could find no evidence of gene amplification or rearrangement of this gene in any tumour. The significance of activation of ras oncogenes in the pathogenesis of human bladder carcinoma is unclear.