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Clinical Evaluation of the Heterologous Oocyte Penetration (HOP) Test
Author(s) -
SUTHERLAND P. O.,
MATSON P. L.,
MOORE H. D. M.,
GOSWAMY R.,
PARSONS J. H.,
VAID P.,
PRYOR J. P.
Publication year - 1985
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1985.tb06432.x
Subject(s) - oocyte , hamster , andrology , hop (telecommunications) , heterologous , zona pellucida , biology , epididymis , embryo , sperm , medicine , microbiology and biotechnology , genetics , gene , computer science , computer network
Summary— The heterologous oocyte penetration (HOP) test, using zona‐free hamster oocytes, was used to assess the fertilising capacity of human spermatozoa. There was good correlation between the ability of ejaculated spermatozoa to penetrate the zona‐free hamster oocytes and intact human oocytes. Using epididymal spermatozoa, the HOP test results showed that the ability to penetrate oocytes was acquired during their passage through the epididymis. Applied clinically, the HOP test enables a group of infertile men to be identified with a functional defect of their spermatozoa; these men may not be identified at routine seminal fluid analysis. Men with two negative HOP test results were confirmed as being infertile since their wives, if normal, conceived rapidly when donor spermatozoa were artificially inseminated.