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Choosing the Right Intravesical Chemotherapeutic Agent. Results of an In vitro Monolayer Cell Culture Assay
Author(s) -
WHELAN P.,
FLYNN C.,
RAMSDEN C.
Publication year - 1984
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1984.tb06139.x
Subject(s) - thiotepa , clonogenic assay , mitomycin c , monolayer , in vitro , trypsinization , chemistry , cell culture , cancer research , microbiology and biotechnology , biology , pharmacology , medicine , chemotherapy , surgery , biochemistry , cyclophosphamide , trypsin , genetics , enzyme
Summary— The use of a monolayer cell culture technique is described. Twenty‐four superficial bladder tumours have been tested against Thiotepa, Adriamycin and Mitomycin C. Cultures were set up in parallel for a clonogenic assay after the method of Salmon. In the monolayer all 24 tumours grew; 21 showed a greater than 50% inhibition to Mitomycin, 13 to Adriamycin and 6 to Thiotepa. In the clonogenic assays, only 12 grew satisfactorily. It is concluded that this monolayer assay gives a reasonable prediction of the sensitivities of the commonly used intravesical agents.