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Experimental Trypsin‐induced Urothelial Cell Separation in vivo
Author(s) -
GARDINER R. A.,
MASTERS J. R. W.,
MOLLAND ELIZABETH A.
Publication year - 1980
Publication title -
british journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.773
H-Index - 148
eISSN - 1464-410X
pISSN - 0007-1331
DOI - 10.1111/j.1464-410x.1980.tb08920.x
Subject(s) - trypsin , in vivo , urothelial cell , regeneration (biology) , enzyme , catheter , chemistry , pathology , urology , cell , cancer research , urinary bladder , urothelium , medicine , biology , microbiology and biotechnology , biochemistry , surgery
Summary— A morphological study of the effects of different concentrations of trypsin on the normal rat bladder in vivo is described. A low concentration (5000 u/ml) of the pure enzyme caused extensive urothelial cell separation following intravesical instillation via a catheter for 30 min. Urothelial regeneration commenced within 12 h and the appearance of the bladder was normal within 3 months. However, the development of submucosal haemorrhage and ulceration, partly as a result of infection, indicates that further experimental work is required before trypsin can be evaluated clinically for removing in situ carcinoma or reducing tumour bulk. The enzyme might also be used to produce more cellular material for cytological examination.