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Autoradiographic Investigations on Cell Proliferation in the Digestive Mucosa of Fishes
Author(s) -
Kiss Robert,
Launoit Yvan,
Lenglet Georges,
Danguy Andre
Publication year - 1988
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1988.tb00919.x
Subject(s) - biology , mucus , thymidine , labelling , epithelium , stomach , intestinal mucosa , anatomy , pathology , medicine , biochemistry , in vitro , ecology , genetics
The labelling index (TLI) of the digestive mucosa of some fish species was determined following a pulse labelling with tritiated thymidine ([ 3 H]TdR) and light microscopic autoradiography. In the oesophageal epithelium, proliferation was observed to occur in non mucus‐secreting cells. In the intestine, both undifferentiated and absorptive cells incorporated [ 3 H)TdR within 1 h after injection. Statistically significant differences in [ 3 H]TdR incorporation were observed between the upper intestine region and both the middle and lower parts on the one hand, and between the middle and lower parts on the other hand. Mucus‐secreting cells seemed unable to proliferate. In the stomach, significantly fewer labelled nuclei were counted; they were located in the isthmus epithelium. No significant difference was observed between the TLI of these regions in the different species.