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In vitro Excystation of Metacercariae of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae)
Author(s) -
Kalantan A. M. N.,
Arfin M.
Publication year - 1988
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1988.tb00913.x
Subject(s) - digenea , biology , pepsin , trypsin , in vitro , trematoda , helminths , enzyme , biochemistry , zoology
In vitro excystation of metacercariae of Clinostomum complanatum (Rudolphi, 1819) was studied. Excystation was an active process, initiated by a rise in temperature. The metacercariae excysted in enzymic as well as non enzymic medium, but the rate of excystation was affected by the temperature and/or pH of the medium. The optimal excystation was obtained in pepsin‐Tyrode at pH 2.3‐3.5 and a temperature of 42°C. Trypsin treatment was not required.