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Extracellular Matrix in Platyhelminths, with Special Reference to the Presence of Fibronectin
Author(s) -
Lindroos Paula,
Wikgren Marianne
Publication year - 1987
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1987.tb00885.x
Subject(s) - basal lamina , biology , fibronectin , extracellular matrix , staining , extracellular , matrix (chemical analysis) , immunofluorescence , connective tissue , peroxidase , anatomy , microbiology and biotechnology , pathology , ultrastructure , antibody , immunology , biochemistry , enzyme , chemistry , medicine , genetics , chromatography
The extracellular matrix (ECM) was studied in the turbellarians Polycelis nigra and Microstomum lineare , the cestode Diphyllobothrium dendriticum and the trematode Dicrocoelium dendriticum. Routine LM staining methods for connective tissue gave positive results only in P. nigra. Positive staining reactions were observed in the subepithelial basal lamina, around the pharynx and as strings in the tissues. Peroxidase‐anti‐peroxidase and immunofluorescence methods were used to identify fibronectin. Positive results were obtained in all species. Positive reactivity to anti‐fibronectin was observed in the subepithelial basal lamina and as a thin network between the cells of the tissues. Some intracellular reactivity occurred, but the cell type was not identified. In M. lineare a strong positive reactivity was observed in the regenerating area.