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Effect of Cytochalasin B on Eggs of Atlantic Salmon and Rainbow Trout
Author(s) -
Bolla Sylvie,
Refstie Terje
Publication year - 1985
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1985.tb00837.x
Subject(s) - rainbow trout , biology , ploidy , polyploid , cytochalasin b , zoology , chromosome , embryo , cytochalasin , karyotype , flow cytometry , fishery , genetics , fish <actinopterygii> , cell , gene , cytoskeleton
Cytochalasin B action has been investigated in polyploidisation and gynogenesis in Atlantic salmon and rainbow trout. Solutions of 10, 20, 30 and 50 mg/1 were used to treat eggs from 35 h° to the 2‐, 4‐ and 8‐cell stages for rainbow trout, from 35 h° to the 2‐ and 4‐cell stages, and 50 h° to the 8‐cell stage for salmon. Survival rate was low in all treated groups. Low frequencies of polyploid and gynogenetic individuals were observed in embryos and alevins by means of two methods: DNA analysis by flow cytometry and chromosome counting. All polyploids observed were triploids without evidence of mosaicism. A few spontaneous diploid gynogenetic rainbow trout were registrated, as well as spontaneous triploid salmon, in controls.