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Histochemical Observations on Carbohydrates in Connective Tissue Structures and Basement Membranes of Hemi‐ and Cephalochordates
Author(s) -
Welsch U.,
Schumacher U.
Publication year - 1984
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1984.tb00815.x
Subject(s) - wheat germ agglutinin , griffonia simplicifolia , connective tissue , basement membrane , biology , lectin , soybean agglutinin , concanavalin a , microbiology and biotechnology , anatomy , biochemistry , genetics , in vitro
Connective tissue components and light microscopical basement membranes of Saccoglossus horsti (Enteropneusta, Hemichordata) and Branchiostoma lanceolatum have been studied with Aldehyde Fuchsin, the PAS‐reaction, Alcian Blue (pH 0.2) and fluorescein conjugated (FITC) lectins: concanavalin A (Con A), wheat germ agglutinin (WGA), soy bean agglutinin (SBA), leucoagglutinin (LA), Griffonia (Bandeiraea) simplicifolia agglutinin (GSA I), Griffonia (Bandeiraea) simplicifolia isolectin B 4 (GS I B4). In Saccoglossus and Branchiostoma , both the PAS‐reaction and Alcian blue give a good general survey over the distribution of the principal basement membranes and connective tissue structures. Lectin binding proved less intensive in Saccoglossus than in Branchiostoma , in which FITC‐Con A, FITC‐GSA and FITC‐WGA react strongly with the dermal (especially in the metapleural folds) and axial connective tissue, as well as the myosepta, the gill arch skeleton and numerous basement membranes. Con A outlines distinctly the major blood vessels in the pharyngeal area. Con A, WGA, GSA and GSI B 4 are markers for basement membranes.