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The Study of Prolactin in the Pituitary Gland of the Atlantic Eel ( Anguilla anguilla ) and the Atlantic Salmon ( Salmo salar ) by Immunofluorescence Technique
Author(s) -
Aler Gudrun M.
Publication year - 1971
Publication title -
acta zoologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.414
H-Index - 37
eISSN - 1463-6395
pISSN - 0001-7272
DOI - 10.1111/j.1463-6395.1971.tb00554.x
Subject(s) - salmo , prolactin , biology , immunofluorescence , brackish water , salt water , fresh water , fishery , antibody , zoology , fluorescein , pituitary gland , endocrinology , fluorescence , medicine , salinity , hormone , fish <actinopterygii> , ecology , oceanography , immunology , physics , quantum mechanics , geology
The prolactin containing cells in the Atlantic eel and the Atlantic salmon have been localized by fluorescein‐labelled antibodies to ovine prolactin. Two fluorescence‐methods have been used: the direct and the indirect. Erythrosinophilic cells in the follicles of the rostral pars distalis were stained by the fluorescent antibodies in both eel and salmon. Eels from brackish water were transferred to fresh or salt water and differences in the contents of prolactin in the hypophyses were tested. Attempts were made at a semi‐quantitative estimation of prolactin, using a fluorescent antibody technique. Eels, which had been kept for three days in fresh water contained a smaller quantity of prolactin in their hypophyses, than the eels which were kept in salt water.