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Proliferation of Streptococcus zooepidemicus and Pseudomonas aeruginosa within a simulated subpalpebral lavage flushed with equine serum
Author(s) -
Jacobi Susan,
Townsend Wendy M.,
Bolin Carole A.
Publication year - 2009
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/j.1463-5224.2009.00725.x
Subject(s) - pseudomonas aeruginosa , microbiology and biotechnology , saline , incubation , microbiological culture , catheter , streptococcus equi , inoculation , andrology , chemistry , medicine , bacteria , biology , surgery , pathology , anesthesia , biochemistry , genetics
Objective To evaluate whether equine serum administered via a simulated subpalpebral lavage system (SPL) supports proliferation of Streptococcus zooepidemicus or Pseudomonas aeruginosa within the tubing. Procedures A sterile i.v. catheter with injection cap was inserted into sterilized silicone tubing (Mila ® ). To mimic an SPL within the dorsal conjunctival fornix, the tubing was secured to an elevated platform. The tip of the tubing extended from the platform into a vial containing culture medium just inoculated with approximately 1.5 × 10 8 CFU/mL P. aeruginosa or S. zooepidemicus . To mimic administration of medication, the tubing was infused twice daily with equine serum, sterile saline (negative control), or culture medium (positive control) followed by air. Incubation was at 25 or 37 °C. At 24, 48, and 72 h postinoculation, samples were obtained for bacterial culture from one simulated SPL for each experimental variant. The following sections were cultured: (i) tubing tip previously submerged in the inoculated culture medium, (ii) tubing mid‐section, and (iii) tip of the i.v. catheter. The experiment was performed in triplicate. Results Streptococcus zooepidemicus or P. aeruginosa were isolated from 100% of the tubing tips. Streptococcus zooepidemicus was isolated from one mid‐section flushed with culture medium incubated at 37 °C. All other samples were negative for growth of the inoculated agents. Conclusions Streptococcus zooepidemicus and P. aeruginosa did not proliferate within silicone tubing infused with equine serum. These data suggest that topical serum can be safely administered through a superiorly placed SPL in clinical cases.