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Perinatal hypoxia induces subsequent retinal degeneration in the offspring of ovoviviparous fish, Xiphophorous maculates
Author(s) -
Lü LanHai,
Li JiCheng,
Wai Maria Sen Mun,
Lam Wai Ping,
Forster E. Lucy,
Fang MaRong,
Yew David T.
Publication year - 2007
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/j.1463-5224.2007.00552.x
Subject(s) - offspring , retinal , biology , hatching , retina , macular degeneration , hypoxia (environmental) , tunel assay , fish <actinopterygii> , anatomy , apoptosis , ophthalmology , medicine , zoology , neuroscience , pregnancy , oxygen , chemistry , genetics , biochemistry , fishery , organic chemistry
Objective This experiment evaluated the perinatal hypoxic effect on the retina of offspring of the ovoviviparous fish. Animal studied The ovoviviparous fish Xiphophorous maculates was used for the experiment. Procedure The mothers were kept in a hypoxic environment of 3.5% oxygen for 6 h, starting 30 h before hatching. Subsequently, the retinae of the offspring were fixed, sectioned at 6 µm and evaluated microscopically from the age of 1 to 35 days. Results Degeneration of the outer nuclear layer of the retina was noted on the 3rd day and severe retinal degeneration was observed on the 35th day. Immunocytochemistry confirmed apoptosis by TUNEL reaction. There was no difference in neovascularization, as revealed by vascular endothelial growth factor, between controls (group 1) and hypoxic fish (group 2). Conclusions Perinatal hypoxia could have long‐lasting effects on the central nervous system in some species.