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Evaluation of canine serum for the presence of antiretinal autoantibodies in sudden acquired retinal degeneration syndrome
Author(s) -
Keller Rachel L.,
Kania Stephen A.,
Hendrix Diane V. H.,
Ward Daniel A.,
Abrams Kenneth
Publication year - 2006
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/j.1463-5224.2006.00466.x
Subject(s) - antibody , western blot , autoantibody , antigen , pathology , retinal , horseradish peroxidase , microbiology and biotechnology , blot , biology , immunoassay , enzyme , chemistry , medicine , immunology , biochemistry , gene
Abstract Objective To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme‐linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures All serum samples were subjected to ELISA with saline‐soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive controls for both procedures. Affinity‐purified goat antidog IgG and IgM labeled with horseradish peroxidase were used for all clinical samples and goat antirabbit IgG was used as the secondary antibody for the positive controls. Results ELISA demonstrated antibody reaction with all samples. Western blot immunoassays identified multiple bands in all canine serum samples, as well as in negative controls. Approximate sizes of the bands were 25 and 50 kDa, corresponding to IgG light and heavy chains, respectively. Conclusion No antiretinal autoantibodies were identified in the serum of dogs affected by SARDS as compared to normal canine patients.