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Metabolism of high density lipoprotein apolipoprotein A‐I and cholesteryl ester in insulin resistant dog: a stable isotope study
Author(s) -
Briand F.,
Bailhache E.,
Nguyen P.,
Krempf M.,
Magot T.,
Ouguerram K.
Publication year - 2007
Publication title -
diabetes, obesity and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.445
H-Index - 128
eISSN - 1463-1326
pISSN - 1462-8902
DOI - 10.1111/j.1463-1326.2006.00588.x
Subject(s) - medicine , endocrinology , scavenger receptor , insulin resistance , chemistry , apolipoprotein b , reverse cholesterol transport , cholesteryl ester , insulin , metabolism , high density lipoprotein , cholesterol , catabolism , cholesterylester transfer protein , lipoprotein , biology
Aims: In reverse cholesterol transport (RCT), hepatic Scavenger Receptor class B type I (SR‐BI) plays an important role by mediating the selective uptake of high‐density lipoprotein cholesteryl ester (HDL‐CE). However, little is known about this antiatherogenic mechanism in insulin resistance. HDL‐CE selective uptake represents the main process for HDL‐CE turnover in dog, a species lacking cholesteryl ester transfer protein activity. We therefore investigate the effects of diet induced insulin resistance on RCT. Methods: Five beagle dogs, in healthy and insulin resistant states, underwent a primed constant infusion of [1,2 13 C 2 ]acetate and [5,5,5‐ 2 H 3 ]leucine, as labelled precursors of CE and apolipoprotein (apo) A‐I, respectively. Data were analysed using modelling methods. Results: HDL‐apo A‐I concentration did not change in insulin resistant state but apo A‐I absolute production rate (APR) and fractional catabolic rate (FCR) were both higher (2.2‐ and 2.4‐fold, respectively, p < 0.05). HDL‐CE levels were lower (1.2‐fold, p < 0.05). HDL‐CE APR and FCR were both lower (2.3‐ and 2‐fold, respectively, p < 0.05), as well as selective uptake (2.6‐fold, p < 0.05). Conclusions: Lower HDL‐CE selective uptake suggests that RCT is impaired in obese insulin resistant dog.