Premium
Combination treatment of vitamin C and desferrioxamine suppresses glomerular superoxide and prostaglandin E 2 production in diabetic rats
Author(s) -
Iino K.,
Iwase M.,
Sonoki K.,
Yoshinari M.,
Iida M.
Publication year - 2005
Publication title -
diabetes, obesity and metabolism
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.445
H-Index - 128
eISSN - 1463-1326
pISSN - 1462-8902
DOI - 10.1111/j.1463-1326.2005.00371.x
Subject(s) - medicine , endocrinology , diabetic nephropathy , renal function , nephropathy , creatinine , streptozotocin , vitamin c , superoxide , vitamin e , prostaglandin e , glomerular hyperfiltration , diabetes mellitus , chemistry , antioxidant , biochemistry , enzyme
Aims: Increased oxidative stress may contribute to the development of diabetic nephropathy. Conversely, it has been proposed that enhanced glomerular production of prostaglandin E 2 (PGE 2 ) may be the cause of glomerular hyperfiltration in streptozotocin (STZ)‐induced diabetic rats. As the role of superoxide anion (O 2– ) production in early diabetic nephropathy is not fully understood, we investigated the effect of vitamin C and desferrioxamine treatment on glomerular O 2– and PGE 2 production in diabetic rats. Methods: STZ‐induced diabetic rats were given drinking water containing 1 g/l of vitamin C and desferrioxamine for 10 days, and glomerular O 2– production, glomerular PGE 2 synthesis and creatinine clearance were examined. Results: Glomerular O 2– production increased in untreated diabetic rats compared to non‐diabetic controls (142.2 ± 12.4 vs. 65.4 ± 3.6 counts/mg protein/min). Treatment with vitamin C and desferrioxamine significantly decreased glomerular O 2– production (93.7 ± 6.7 counts/mg protein/min). Glomerular PGE 2 synthesis and creatinine clearance were significantly increased in untreated diabetic rats compared to controls and PGE 2 synthesis was reduced and creatinine clearance tended to decrease by the treatment. Conclusions: Our results demonstrated that vitamin C and desferrioxamine suppressed the enhanced glomerular O 2– production with subsequent decrease in PGE 2 production. Antioxidant therapy may be beneficial in preventing the development of diabetic nephropathy.