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Determination of 2‐aminoethanol in 2,2’,2’’‐nitrilotriethanol by reversed‐phase HPLC‐UV using o ‐phthalaldehyde as a pre‐column derivatizing reagent
Author(s) -
Kageshima K.,
Okada K.,
Saito H.,
Shimizu T.
Publication year - 2005
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1463-1318.2005.00273_2.x
Subject(s) - high performance liquid chromatography , chromatography , calibration curve , reagent , chemistry , detection limit , analytical chemistry (journal) , derivatization , o phthalaldehyde , standard curve , organic chemistry
A measurement method for determination of 2‐aminoethanol in 2,2’,2’’‐nitrilotriethanol by reversed‐phase high‐performance liquid chromatography (HPLC) with an ultraviolet detector has been developed using o ‐phthalaldehyde as a pre‐column derivatizing reagent. As a result, the calibration curve of 2‐aminoethanol standard solutions was linear over the range of 1.0 × 10 – 5 – 5.0 × 10 – 3 mol L –1 with a correlation coefficient of 0.996 and the detection limit, defined as S / N = 3, was 7.5 × 10 – 7 mol L –1 . The relative SD at 5.0 × 10 – 5 mol/ L –1 of 2‐aminoethanol standard solution was 6.0% ( n = 6). This measurement method was applied to the determination of 2‐aminoethanol in several commercial 2,2’,2’’‐nitrilotriethanol samples and it was found that both the calibration curve method and the standard addition method based on the peak height are available for the determination of 2‐aminoethanol. These results suggest that the method reported here will be useful for determination of 2‐aminoethanol in 2,2’,2’’‐nitrilotriethanol.