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Sulforaphane inhibits growth of a colon cancer cell line
Author(s) -
Frydoonfar H. R.,
McGrath D. R.,
Spigelman A. D.
Publication year - 2004
Publication title -
colorectal disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.029
H-Index - 89
eISSN - 1463-1318
pISSN - 1462-8910
DOI - 10.1111/j.1463-1318.2004.00488.x
Subject(s) - sulforaphane , cruciferous vegetables , colorectal cancer , medicine , isothiocyanate , cell growth , phytochemical , cancer research , cell culture , cancer , growth inhibition , oncology , biochemistry , traditional medicine , biology , genetics
Objective The consumption of cruciferous vegetables has a protective effect on the development of colorectal cancer. The phytochemical Sulforaphane is an isothiocyanate found almost exclusively in cruciferous vegetables. We have studied the effect of Sulforaphane on cell proliferation of an HT‐29 colon cancer cell line. Materials and methods HT‐29 colon cancer cells were cultured in 96‐well microtitre plates. Sulforaphane (in concentrations ranging from 0.01 to 0.1 mmol) were added to the wells. Cell proliferation was measured using the colourimetric assay technique. Results The proliferation of colon cancer cells was significantly reduced by Sulforaphane at concentrations of ≥0.02 mmol. Conclusion These findings may help explain the epidemiologically proven protective effect of vegetables against colon cancer.