Open AccessThe proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65
Author(s) -
Kucknoor Ashwini S.,
Mundodi Vasanthakrishna,
Alderete John F.
Publication year - 2007
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/j.1462-5822.2007.00979.x
Subject(s) - biology , secretory protein , bacterial adhesin , trichomonas vaginalis , secretion , gene expression , microbiology and biotechnology , heat shock protein , gene , biochemistry , virulence
Summary We showed recently that contact of human vaginal epithelial cells (VECs) by Trichomonas vaginalis and incubation with trichomonad proteins in conditioned medium induced expression of VEC genes. We performed 2‐D SDS‐PAGE followed by MALDI‐TOF to identify the major secreted proteins. Based on protein abundance and separation of spots in 2‐D gels, 32 major secreted proteins were examined, which gave 19 proteins with accession numbers. These proteins included known secreted cysteine proteinases. In addition, other secreted proteins were enzymes of carbohydrate metabolism, adhesin protein AP65 , heat shock proteins, thioredoxin reductase and coronins. We confirmed that the secreted trichomonad proteins induced expression of VEC genes, including interleukin 8 (IL‐8), COX‐2 and fibronectin. Purified AP65 added to VECs had a pronounced effect only on IL‐8 gene expression, which was inhibited in the presence of 12G4 monoclonal antibody to AP65 . Moreover, AP65 expressed episomally within epithelial cells was found to enhance the expression of IL‐8 and COX‐2. This may be the first report of analysis of the secreted proteins of T. vaginalis and of the host epithelial cell response to these proteins and to the prominent adhesin AP65 .