Open AccessStaphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is downregulated by cannabinoid receptor 1
Author(s) -
Hu DongLiang,
Zhu Gang,
Mori Fumiaki,
Omoe Katsuhiko,
Okada Motohiro,
Wakabayashi Koichi,
Kaneko Sunao,
Shinagawa Kunihiro,
Nakane Akio
Publication year - 2007
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/j.1462-5822.2007.00957.x
Subject(s) - biology , enterotoxin , serotonin , cannabinoid , receptor , microbiology and biotechnology , pharmacology , biochemistry , escherichia coli , gene
Summary Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus are the most recognizable bacterial superantigenic toxins causing food poisoning in humans throughout the world. However, it remains unclear how SEs induce emesis and its emetic signal pathway. We investigated a mechanism of SEA‐induced emesis using a small emetic animal model, house musk shrew. SEA‐induced emesis in the animals was inhibited by a 5‐hydroxytryptamine (5‐HT) synthesis inhibitor and a 5‐HT 3 receptor antagonist. SEA could increase 5‐HT release in the small intestine. Pre‐treatment with 5,7‐dihydroxytryptamine (5,7‐DHT) markedly inhibited SEA‐induced emesis. SEA‐induced emesis was also abolished by surgical vagotomy. Furthermore, cannabinoid (CB) receptor agonists inhibited SEA‐induced emesis, and the action was reversed by a CB1 antagonist. Both 5‐HT release and CB1 receptor expression were found in the mucosal and myenteric plexus of the intestine. Moreover, a CB1 receptor agonist significantly decreased the 5‐HT release in the intestine. These results demonstrate that SEA induces 5‐HT release in intestine, rather than in brain, and that the 5‐HT 3 receptors on vagal afferent neurons are essential for SEA‐stimulated emesis. In addition, SEA‐induced emesis is downregulated by the CB system through decreasing 5‐HT release in intestine.