The Ag85B protein of Mycobacterium tuberculosis may turn a protective immune response induced by Ag85B‐DNA vaccine into a potent but non‐protective Th 1 immune response in mice

Summary Clarifying how an initial protective immune response to tuberculosis may later loose its efficacy is essential to understand tuberculosis pathology and to develop novel vaccines. In mice, a primary vaccination with Ag85B‐encoding plasmid DNA (DNA‐85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B‐specific CD4 + T cells producing IFN‐γ and controlling intramacrophagic MTB growth. Surprisingly, this protection was eliminated by Ag85B protein boosting. Loss of protection was associated with a overwhelming CD4 + T cell proliferation and IFN‐γ production in response to Ag85B protein, despite restraint of Th 1 response by CD8 + T cell‐dependent mechanisms and activation of CD4 + T cell‐dependent IL‐10 secretion. Importantly, these Ag85B‐responding CD4 + T cells lost the ability to produce IFN‐γ and control MTB intramacrophagic growth in coculture with MTB‐infected macrophages, suggesting that the protein‐dependent expansion of non‐protective CD4 + T cells determined dilution or loss of the protective Ag85B‐specific CD4 + induced by DNA‐85B vaccination. These data emphasize the need of exerting some caution in adopting aggressive DNA‐priming, protein‐booster schedules for MTB vaccines. They also suggest that Ag85B protein secreted during MTB infection could be involved in the instability of protective anti‐tuberculosis immune response, and actually concur to disease progression.