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The blue light receptor complex WC ‐1/2 of S chizophyllum commune is involved in mushroom formation and protection against phototoxicity
Author(s) -
Ohm Robin A.,
Aerts David,
Wösten Han A. B.,
Lugones Luis G.
Publication year - 2013
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2012.02878.x
Subject(s) - dikaryon , biology , mutant , gene , neurospora crassa , mushroom , microbiology and biotechnology , botany , genetics
Summary Blue light is necessary for initiation of mushroom formation in S chizophyllum commune . The genome of this basidiomycete contains homologues of the blue light receptor genes wc‐1 and wc‐2 of N eurospora crassa . Here, it is shown that inactivation of either or both of these genes in S . commune results in a blind phenotype. Mushroom formation was abolished in dikaryons and they formed symmetrical instead of asymmetrical colonies. Development was restored in a temperature dependent way in a Δ wc‐2 Δ wc‐2 strain by introducing a construct encompassing the wc‐2 gene under control of the promoter of the heat shock gene hsp3 . A genome‐wide expression analysis showed that the transcription factor genes c2h2 and hom1 as well as many hydrophobin genes are downregulated in light‐grown colonies of the Δ wc‐2 Δ wc‐2 mutant when compared with the wild‐type dikaryon. Inactivation of wc‐1 and/or wc‐2 also resulted in sensitivity of the mycelium to intense light. Monokaryotic mutant strains only survived exposure to 6500 lux of light by growing into the agar. Expression analysis indicates that the photosensitivity of the Δ wc‐1 and Δ wc‐2 strains is due to lower levels of photolyase and ferrochelatase.