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Bacteria rather than Archaea dominate microbial ammonia oxidation in an agricultural soil
Author(s) -
Jia Zhongjun,
Conrad Ralf
Publication year - 2009
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2009.01891.x
Subject(s) - archaea , biology , bacteria , soil microbiology , stable isotope probing , nitrogen cycle , ammonia , microorganism , ammonia monooxygenase , microcosm , ammonium , thaumarchaeota , nitrification , environmental chemistry , ecology , biochemistry , nitrogen , chemistry , genetics , organic chemistry
Summary Agricultural ecosystems annually receive approximately 25% of the global nitrogen input, much of which is oxidized at least once by ammonia‐oxidizing prokaryotes to complete the nitrogen cycle. Recent discoveries have expanded the known ammonia‐oxidizing prokaryotes from the domain Bacteria to Archaea . However, in the complex soil environment it remains unclear whether ammonia oxidation is exclusively or predominantly linked to Archaea as implied by their exceptionally high abundance. Here we show that Bacteria rather than Archaea functionally dominate ammonia oxidation in an agricultural soil, despite the fact that archaeal versus bacterial amoA genes are numerically more dominant. In soil microcosms, in which ammonia oxidation was stimulated by ammonium and inhibited by acetylene, activity change was paralleled by abundance change of bacterial but not of archaeal amoA gene copy numbers. Molecular fingerprinting of amoA genes also coupled ammonia oxidation activity with bacterial but not archaeal amoA gene patterns. DNA‐stable isotope probing demonstrated CO 2 assimilation by Bacteria rather than Archaea . Our results indicate that Archaea were not important for ammonia oxidation in the agricultural soil tested.