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Ammonia‐dependent differential regulation of the gene cluster that encodes ammonia monooxygenase in Nitrosococcus oceani ATCC 19707
Author(s) -
El Sheikh Amal F.,
Klotz Martin G.
Publication year - 2008
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2008.01766.x
Subject(s) - ammonia monooxygenase , biology , operon , promoter , rpon , gene , sigma factor , gene cluster , ammonia , genetics , microbiology and biotechnology , biochemistry , gene expression , escherichia coli , archaea
Summary Molecular analysis of the ammonia monooxygenase‐encoding gene cluster in Nitrosococcus oceani revealed that the amo genes are differentially expressed from three promoters dependent on the external ammonia concentration. Whereas a distal amoRCABD operon promoter, pC1, is active only in the presence of ammonia, identified proximal amoC (pC2) and amoA (pA) sigma‐70‐type promoters are constitutive. Promoter region pC2 also contains a consensus sequence for binding of RpoN, usually involved in nitrogen starvation response. Additional regulation of transcript stability by anti‐termination and mRNA degradation are proposed and discussed.