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Phylogenetic analysis of Porphyromonas species isolated from the oral cavity of Australian marsupials
Author(s) -
Mikkelsen Deirdre,
Milinovich Gabriel J.,
Burrell Paul C.,
Huynh Sharnan C.,
Pettett Lyndall M.,
Blackall Linda L.,
Trott Darren J.,
Bird Philip S.
Publication year - 2008
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2008.01668.x
Subject(s) - biology , marsupial , porphyromonas gingivalis , phylogenetic tree , zoology , monophyly , phylogenetics , brushtail possum , 16s ribosomal rna , microbiology and biotechnology , genetics , gene , clade , bacteria
Summary Porphyromonas species are frequently isolated from the oral cavity and are associated with periodontal disease in both animals and humans. Black, pigmented Porphyromonas spp. isolated from the gingival margins of selected wild and captive Australian marsupials with varying degrees of periodontal disease (brushtail possums, koalas and macropods) were compared phylogenetically to Porphyromonas strains from non‐marsupials (bear, wolf, coyote, cats and dogs) and Porphyromonas gingivalis strains from humans using 16S rRNA gene sequence analysis. The results of the phylogenetic analysis identified three distinct groups of strains. A monophyletic P. gingivalis group (Group 1) contained only strains isolated from humans and a Porphyromonas gulae group (Group 2) was divided into three distinct subclades, each containing both marsupial and non‐marsupial strains. Group 3, which contained only marsupial strains, including all six strains isolated from captive koalas, was genetically distinct from P. gulae and may constitute a new Porphyromonas species.