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Identification and analysis of a glutaryl‐CoA dehydrogenase‐encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB
Author(s) -
Blázquez Blas,
Carmona Manuel,
García José Luis,
Díaz Eduardo
Publication year - 2008
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2007.01468.x
Subject(s) - biology , pseudomonas putida , gene , genetics , promoter , mutant , regulator gene , regulation of gene expression , gene expression
Summary In this work, the gcdH gene from the denitrifying β‐proteobacterium Azoarcus sp. CIB was shown to encode a glutaryl‐CoA dehydrogenase, which is essential for the anaerobic catabolism of many aromatic compounds and some alicyclic and dicarboxylic acids. The primary structure of the GcdH protein is highly conserved in many organisms. The divergently transcribed gcdR gene, encoding a LysR‐type transcriptional regulator, accounts for the glutaconate/glutarate‐specific activation of the Pg promoter driving expression of gcdH . The Azoarcus sp. CIBd gcdH mutant strain harbouring a disrupted gcdH gene was used as host to identify heterologous gcdH genes, such as that from Pseudomonas putida KT2440. Moreover, the expression of gcdH from P. putida can be efficiently controlled by the GcdR activator in Azoarcus sp. CIB, demonstrating the existence of cross‐talk between GcdR regulators and gcdH promoters from members of different phylogenetic subgroups of proteobacteria.