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Populations related to Alkanindiges , a novel genus containing obligate alkane degraders, are implicated in biological foaming in activated sludge systems
Author(s) -
Klein Adam N.,
Frigon Dominic,
Raskin Lutgarde
Publication year - 2007
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2007.01307.x
Subject(s) - biology , gammaproteobacteria , 16s ribosomal rna , activated sludge , terminal restriction fragment length polymorphism , ribosomal rna , oligomer restriction , phylogenetic tree , acinetobacter , microbiology and biotechnology , restriction fragment length polymorphism , bacteria , gene , oligonucleotide , sewage treatment , genetics , polymerase chain reaction , engineering , waste management
Summary Activated sludge mixed liquor and biological foam samples were collected from five full‐scale municipal wastewater treatment plants in Illinois, all of which were exhibiting biological foaming at the time of sampling. Oligonucleotide probe hybridization consistently measured higher levels of Gammaproteobacteria rRNA in the foam as compared with the mixed liquor for all treatment plants analysed. Cloning and sequencing of 16S rRNA gene amplicons led to the identification of populations which were abundant in each of the treatment plants. These populations were related to the Alkanindiges/Acinetobacter cluster within the Gammaproteobacteria . Further analysis of the 16S rRNA sequences indicated that they clustered in three phylogenetic groups outside the main Alkanindiges/Acinetobacter cluster, suggesting that these groups may represent new taxa. Terminal‐restriction fragment length polymorphism analysis showed that these populations were enriched in the foam compared with the underlying mixed liquor similar to the enrichment of the Gammaproteobacteria measured by oligonucleotide probe membrane hybridization. The observed enrichment in foam samples is suggestive of a role for these populations in foam formation or stabilization, and their presence in all treatment plants analysed in this study may be indicative of their widespread abundance in foaming plants.