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The use of functional genomics for the identification of a gene cluster encoding for the biosynthesis of an antifungal tambjamine in the marine bacterium Pseudoalteromonas tunicata
Author(s) -
Burke Catherine,
Thomas Torsten,
Egan Suhelen,
Kjelleberg Staffan
Publication year - 2007
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2006.01177.x
Subject(s) - biology , gene cluster , identification (biology) , pseudoalteromonas , gene , antifungal , computational biology , genetics , microbiology and biotechnology , ecology , 16s ribosomal rna
Summary A large insert library was created in Escherichia coli from the DNA of the surface‐associated marine bacterium Pseudoalteromonas tunicata. Screening of the library for antifungal activity resulted in the detection and identification of a large gene cluster encoding for the biosynthesis of an antifungal tambjamine. A biosynthetic pathway has been proposed based on analysis and annotation of the gene cluster.