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Impact of carbon metabolism on 13 C signatures of cyanobacteria and green non‐sulfur‐like bacteria inhabiting a microbial mat from an alkaline siliceous hot spring in Yellowstone National Park (USA)
Author(s) -
Van Der Meer Marcel T. J.,
Schouten Stefan,
Damsté Jaap S. Sinninghe,
Ward David M.
Publication year - 2007
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2006.01165.x
Subject(s) - cyanobacteria , phototroph , biology , botany , heterotroph , photosynthesis , hot spring , algae , total inorganic carbon , isotopes of carbon , microbial mat , sulfur , environmental chemistry , bicarbonate , mixotroph , bacteria , ecology , carbon dioxide , chemistry , total organic carbon , paleontology , genetics , organic chemistry , endocrinology
Summary Alkaline siliceous hot spring microbial mats in Yellowstone National Park are composed of two dominant phototropic groups, cyanobacteria and green non‐sulfur‐like bacteria (GNSLB). While cyanobacteria are thought to cross‐feed low‐molecular‐weight organic compounds to support photoheterotrophic metabolism in GNSLB, it is unclear how this could lead to the heavier stable carbon isotopic signatures in GNSLB lipids compared with cyanobacterial lipids found in previous studies. The two groups of phototrophs were separated using percoll density gradient centrifugation and subsequent lipid and stable carbon isotopic analysis revealed that we obtained fractions with a ∼60‐fold enrichment in cyanobacterial and an approximately twofold enrichment in GNSLB biomass, respectively, compared with the mat itself. This technique was used to study the diel cycling and 13 C content of the glucose pools in and the uptake of 13 C‐bicarbonate by the cyanobacteria and GNSLB, as well as the transfer of incorporated 13 C from cyanobacteria to GNSLB. The results show that cyanobacteria have the highest bicarbonate uptake rates and accumulate glucose during the afternoon in full light conditions. In contrast, GNSLB have relatively higher bicarbonate uptake rates compared with cyanobacteria in the morning at low light levels. During the night GNSLB take up carbon that is likely derived through fermentation of cyanobacterial glucose enriched in 13 C. The assimilation of 13 C‐enriched cyanobacterial carbon may thus lead to enriched 13 C‐contents of GNSLB cell components.