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Molecular characterization of Vibrio harveyi bacteriophages isolated from aquaculture environments along the coast of India
Author(s) -
Shivu Mockshanath M.,
Rajeeva Bettada C.,
Girisha Shivani K.,
Karunasagar Indrani,
Krohne Georg,
Karunasagar Iddya
Publication year - 2007
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1111/j.1462-2920.2006.01140.x
Subject(s) - vibrio harveyi , biology , lytic cycle , myoviridae , siphoviridae , aquaculture , microbiology and biotechnology , restriction fragment length polymorphism , shrimp , rapd , vibrio , genomic dna , bacteriophage , dna , virology , genetics , bacteria , gene , ecology , virus , fishery , polymerase chain reaction , fish <actinopterygii> , genetic diversity , escherichia coli , population , demography , sociology
Summary Seven bacteriophages specific to Vibrio harveyi , the causative agent of luminous vibriosis in shrimp, were isolated from coastal aquaculture systems like shrimp farms, hatcheries and tidal creeks along the east and west coast of India. All the seven phages were found to have the typical head and tail morphology with double‐stranded DNA as genetic material. Morphologically, six phages were grouped under family Siphoviridae and one under Myoviridae . These phages were further characterized with respect to host range, morphology and structural proteins. Genomic fingerprinting was carried out using restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD). Major capsid proteins of all the phages detected by SDS‐PAGE were distinct from one another. All the phages were found to be highly lytic for V. harveyi and had different lytic spectrum for the large number of isolates tested. Six of the seven phages isolated had a broad lytic spectrum and could be potential candidates for biocontrol of V. harveyi in aquaculture systems.